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SRX13413865: RADseq of Chrysemys picta: hatchling female liver
1 ILLUMINA (Illumina HiSeq 2500) run: 1.7M spots, 494M bases, 191.8Mb downloads

Design: We used a restriction site-associated DNA sequencing (RADseq) approach with a single-restriction enzyme, MseI, ligated resulting fragments with two barcode adapters, and pooled samples for size selection. We assessed resulting libraries for quality before paired-end 150 bp sequencing on an Illumina HiSeq platform
Submitted by: Iowa State University
Study: RADseq of painted turtle populations from western USA
show Abstracthide Abstract
In this study, we investigated the population genomic structure of western populations of the widespread painted turtle (Chrysemys picta) to assess genomic evidence of local adaptation and past demography. We wanted to understand the potential role of local adaptation, as assessed through reduced representation sequencing, in the colonization and persistence of populations of painted turtles in the middle and western United States. Populations in this region were previously hypothesized to be the result of a rapid expansion of painted turtles westward following glacier recession and were reported to have few genetic differences among them. We tested this hypothesis and the hypothesis that local adaptation allows populations to survive in the differing environments sampled, and the results inform our understanding of evolutionary patterns in this long-lived, widespread ectotherm that is vulnerable to future climatic conditions.
Sample: NE_59-8
SAMN24045604 • SRS11315813 • All experiments • All runs
Organism: Chrysemys picta
Library:
Name: S474
Instrument: Illumina HiSeq 2500
Strategy: RAD-Seq
Source: GENOMIC
Selection: Restriction Digest
Layout: PAIRED
Runs: 1 run, 1.7M spots, 494M bases, 191.8Mb
Run# of Spots# of BasesSizePublished
SRR172346031,715,220494M191.8Mb2023-10-31

ID:
18520006

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